A new test that detects the pandemic coronavirus in just 5 minutes and don’t require expensive lab equipment. Researchers have used CRISPR gene-editing technology. The new test is the fastest CRISPR-based diagnostic yet. It don’t need a lab, a technician or temperature change. CRISPR tests work by identifying a sequence of RNA, about 20 RNA bases long, that is unique to SARS-CoV-2. The result can be read from a line on the slip of paper. Researchers led by Jennifer Doudna, who won a share of this year’s Nobel Prize in Chemistry yesterday for her co-discovery of CRISPR spent months testing hundreds of guide RNAs to find multiple guides that work in tandem to increase the sensitivity of the test. They add a second guide RNA and can detect as few as 100 viruses per microliter. The test also reveals how much virus a patient has.
However, the conventional coronavirus diagnostic setup, which uses expensive lab-based machines, tracks the virus down to one virus per microliter. Melanie Ott, a virologist at UC San Francisco, helped lead the project with Doudna. The new test is prepared to be offered. Doudna has made fundamental contributions in biochemistry and genetics and received many prestigious awards and fellowships, including the 2020 Nobel Prize in Chemistry. Outside the scientific community, she has been named one of the Time 100 most influential people in 2015